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KMID : 0881720100250040376
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Journal of Food Hygiene and Safety
2010 Volume.25 No. 4 p.376 ~ p.387
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Trends in Rapid Detection Methods for Food-borne pathogenic Microorganisms by Using New Technologies
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Kim Hyun-Joo
Kim Yong-Soo
Chung Myung-Sub
Oh Deog-Hwan
Chun Hyang-Sook
Ha Sang-Do
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Abstract
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Recently, speedy, convenient and easy detection technologies have been developed rapidly and on the contrary, studies on development of traditional detectors applying biochemical characteristics has gradually been decreased. This review examined trend in current studies on detection of food-borne pathogenic microorganisms in the fields of selective media, immuno-assay, Polymerase Chain Reaction (PCR), microarray, terahertz spectroscopy & imagination and so on. Most traditional methods to detect the organisms from food matrix rely on selective media and such a method have disadvantages like long time requirement and distinguishing one species only from each selective medium although they are highly economical. Various new convenient methods such as Enzyme Linked Immuno-sorbent Assay (ELISA), paper-strip kit, fluoroimmunoassay etc. have been developed. The most ideal method for detecting food-borne pathogenic microorganisms in foods should be accurate, convenient, rapid and economical. Additionally, it is needed that capabilities of quantitative analysis and automation to be applied to industries.
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KEYWORD
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Food-borne pathogenic microorganisms, rapid detection, selective media, immuno-assay, microarray
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